Journal: PLoS Pathogens

Article Title: The α-tubulin of Laodelphax striatellus mediates the passage of rice stripe virus (RSV) and enhances horizontal transmission

doi: 10.1371/journal.ppat.1008710

Figure Lengend Snippet: Detection of LsTUB antigen and RSV coat protein (CP) antigen in (A) midgut, (B) hemocytes, and (C) salivary glands of 30 viruliferous adults. Anti-LsTUB and anti-RSV CP monoclonal antibodies were detected using Alexa Fluor 555 (red) or 488 (green) labeled secondary antibodies, respectively. Panels A-A”’ and C-C”’, bar = 100 μm; panels B-B”’ and D-D”’, bar = 50 μm. Abbreviations: sg, salivary glands; psg, principal salivary glands; vm, visceral muscle; and me, midgut epithelium.

Article Snippet: The mouse polyclonal antibody against the RSV CP peptide was produced by HuaAn Biotechnology Co., Ltd (HuaBio, Hangzhou, China).

Techniques: Bioprocessing, Labeling

Journal: PLoS Pathogens

Article Title: The α-tubulin of Laodelphax striatellus mediates the passage of rice stripe virus (RSV) and enhances horizontal transmission

doi: 10.1371/journal.ppat.1008710

Figure Lengend Snippet: (A) Leaves of wild-type N . benthamiana plants were co-agroinfiltrated with a GFP-expressing vector, a vector encoding dsGFP, an empty vector (EV), or a vector encoding P19 (positive control), or NS3, or NS3 mutants (NS3 mut SFL 74-76 AAA and NS3 mut IWI 80-82 AAA ). The leaves were photographed at three days post-infection under UV light. (B) Leaves of wild-type N . benthamiana plants were co-agroinfiltrated with a GFP-expressing vector, a vector encoding dsGFP, and an empty vector (EV), or a vector encoding P19 or NS3 (with or without a vector encoding LsTUB). (C) The expression of LsTUB and RSV NS3 in leaves from different treated spots was determined using anti-LsTUB and anti-RSV NS3 antibodies.

Article Snippet: The mouse polyclonal antibody against the RSV CP peptide was produced by HuaAn Biotechnology Co., Ltd (HuaBio, Hangzhou, China).

Techniques: Expressing, Plasmid Preparation, Positive Control, Infection

Journal: PLoS Pathogens

Article Title: The α-tubulin of Laodelphax striatellus mediates the passage of rice stripe virus (RSV) and enhances horizontal transmission

doi: 10.1371/journal.ppat.1008710

Figure Lengend Snippet: Viruliferous SBPH were fed on artificial diets supplemented with dsGFP or dsTUB. SBPH midgut (A) and salivary glands (B) were analyzed for viral loads by qRT-PCR (red columns indicate dsTUB; blue columns indicate dsGFP). Levels of LsTUB, NS3 and GAPDH were analyzed by immunoblotting, and GAPDH was used as control. Each treatment was replicated three times for qRT-PCR, and values represent means ± SE, A student’s t -test was used to analyze significance; ** represents P <0.01. Midguts and salivary glands of viruliferous SBPH were immunolabeled with anti-RSV NS3 (Alexa Fluor 488, green) and anti-LsTUB (Alexa Fluor 555, red) and then examined by confocal microscopy. Abbreviations: mg, midgut; sg, salivary glands; and vm, visceral muscle. Bar = 50 μm.

Article Snippet: The mouse polyclonal antibody against the RSV CP peptide was produced by HuaAn Biotechnology Co., Ltd (HuaBio, Hangzhou, China).

Techniques: Quantitative RT-PCR, Western Blot, Control, Immunolabeling, Confocal Microscopy

Journal: PLoS Pathogens

Article Title: The α-tubulin of Laodelphax striatellus mediates the passage of rice stripe virus (RSV) and enhances horizontal transmission

doi: 10.1371/journal.ppat.1008710

Figure Lengend Snippet: (A) The effect of LsTUB overexpression and LsTUB knockdown on RSV NS3 in LsST6 transfected Sf9 cells at 24 h after RSV infection. The Sf9 cells were treated with anti-LsTUB antibody labeled with Alexa Fluor 555 (red) and anti-RSV NS3 antibody labeled with Alexa Fluor 488 (green) and then examined by confocal microscopy. Bars = 50 μm. (B) The protein expression level of LsTUB and RSV NS3 were analyzed by immunoblotting. GAPDH was used as control. (C) RSV copy number equivalents were tested by qRT-PCR in Sf9 cells. Total RNA was extracted from Sf9 cells at 1, 6, 12 and 24 h after adding virus particles to analyze the RSV loads. Values represent means ± SE; significance was evaluated by t -test analysis.

Article Snippet: The mouse polyclonal antibody against the RSV CP peptide was produced by HuaAn Biotechnology Co., Ltd (HuaBio, Hangzhou, China).

Techniques: Over Expression, Knockdown, Transfection, Infection, Labeling, Confocal Microscopy, Expressing, Western Blot, Control, Quantitative RT-PCR, Virus

Journal: PLoS Pathogens

Article Title: The α-tubulin of Laodelphax striatellus mediates the passage of rice stripe virus (RSV) and enhances horizontal transmission

doi: 10.1371/journal.ppat.1008710

Figure Lengend Snippet: Typical patterns of LsTUB and RSV NS3 distribution in different organs of dsGFP and dsLsTUB-treated SBPHs at 1 (A, B, I, J, Q, R), 3 (C, D, K, L, S, T), 5 (E, F, M, N, U, V), and 7 days padp (G, H, O, P, W, X). The midgut, hemocytes and salivary glands were labeled with anti-LsTUB antibody conjugated with Alexa Fluor 555 (red) and anti-RSV NS3 antibody conjugated with Alexa Fluor 488 (green) and then examined by confocal microscopy. Bars = 50 μm.

Article Snippet: The mouse polyclonal antibody against the RSV CP peptide was produced by HuaAn Biotechnology Co., Ltd (HuaBio, Hangzhou, China).

Techniques: Labeling, Confocal Microscopy

Journal: Investigative Ophthalmology & Visual Science

Article Title: Connexin50D47A Decreases Levels of Fiber Cell Connexins and Impairs Lens Fiber Cell Differentiation

doi: 10.1167/iovs.13-13188

Figure Lengend Snippet: Levels and immunolocalization of Cx46 in lenses from wild-type and Cx50D47A-expressing mice. (A) Aliquots of lens homogenates from 3.9-month-old mice were subjected to immunoblotting with anti-Cx46 antibodies. (B) Graph represents the densitometric values of the Cx46 bands obtained in three independent experiments expressed as a percentage of the values determined from wild-type littermates. Levels of Cx46 are more severely reduced in homozygous than in heterozygous mice expressing Cx50D47A. (C–E) Photomicrographs show the distribution of immunoreactive Cx46 (green) and nuclei (DAPI, blue) in longitudinal sections of lenses from 1-month-old wild-type (+/+) (C), heterozygous (+/D47A) (D), and homozygous (D47A/D47A) (E) mice. Cx46 has a punctate distribution at appositional membranes in both wild-type and mutant mouse lenses, although its abundance (especially in deeper layers of the lens) is decreased in mutant compared with wild-type mouse lenses. Scale bar: 68 μm.

Article Snippet: 17 Rabbit polyclonal antibodies against a synthetic peptide sequence from within the intracellular loop of mouse Cx46 were generated and affinity purified by YenZym Antibodies, LLC (South San Francisco, CA).

Techniques: Expressing, Western Blot, Mutagenesis